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Pronunciation varies by accent · noun
(biochemistry) A serine protease inhibitor commonly used in the preparation of cell lysates..
One hundred milligrams of frozen lung tissue was homogenized in buffer A (0.32 M sucrose, 5 m M Tris–HCl pH 7.5, 120 m M KCl, 1 m M EDTA, 0.2 m M phenylmethylsulfonylfluoride, 1 μg ml −1 leupeptin, 1 μg ml −1 pepstatin and 1 μg ml −1 aprotinin) and spun at 4 °C, 12 890 g for 10 min.
Use phenylmethylsulfonylfluoride when its meaning, tone and grammar fit the full sentence. A synonym is not always a direct replacement.
Phenylmethylsulfonylfluoride means (biochemistry) A serine protease inhibitor commonly used in the preparation of cell lysates..
Common synonyms include PMSF.
The opposite depends on the specific sense.
One hundred milligrams of frozen lung tissue was homogenized in buffer A (0.32 M sucrose, 5 m M Tris–HCl pH 7.5, 120 m M KCl, 1 m M EDTA, 0.2 m M phenylmethylsulfonylfluoride, 1 μg ml −1 leupeptin, 1 μg ml −1 pepstatin and 1 μg ml −1 aprotinin) and spun at 4 °C, 12 890 g for 10 min.
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