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Pronunciation varies by accent · verb
To attach a biotin residue to a biological macromolecule in order to label it.
After 30 min, the reaction was supplemented to a final volume of 25 μL with 5 units of Klenow fragment (3′→5′ exo-) DNA polymerase (NEB, USA) and 5 μM of biotin-14-dUTP, dATP, dGTP and dCTP and incubated at 37 °C for another 30 min to fill in the overhangs and biotinylate the fragmented DNA.
Use biotinylate when its meaning, tone and grammar fit the full sentence. A synonym is not always a direct replacement.
Biotinylate means To attach a biotin residue to a biological macromolecule in order to label it.
The closest synonym depends on the sentence and intended sense.
The opposite depends on the specific sense.
After 30 min, the reaction was supplemented to a final volume of 25 μL with 5 units of Klenow fragment (3′→5′ exo-) DNA polymerase (NEB, USA) and 5 μM of biotin-14-dUTP, dATP, dGTP and dCTP and incubated at 37 °C for another 30 min to fill in the overhangs and biotinylate the fragmented DNA.
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